| Year: | 1999 |
| Team: | Jacksonville State Gamecocks |
| Sport: | football |
| Conference: | Southland Football League |
| Short Conf: | Southland |
| Record: | 2–9 |
| Conf Record: | 1–6 |
| Head Coach: | Mike Williams |
| Hc Year: | 3rd |
| Hc Games: | first 4 games |
| Head Coach2: | Jeff Richards |
| Hc Games2: | interim, final 7 games |
| Off Coach: | Jeff Richards |
| Oc Year: | 1st |
| Def Coach: | Mike Bugar |
| Dc Year: | 1st |
| Stadium: | Paul Snow Stadium |
The 1999 Jacksonville State Gamecocks football team represented Jacksonville State University as a member of the Southland Football League during the 1999 NCAA Division I-AA football season. The Gamecocks compiled an overall record of 2–9 with a mark of 1–6 in conference play, placing last in the Southland. Jacksonville State played home games at Paul Snow Stadium in Jacksonville, Alabama.