The women's 400 metres hurdles event at the 1999 European Athletics U23 Championships was held in Göteborg, Sweden, at Ullevi on 29 and 30 July 1999.
| Gold | Natasha Danvers | |
| Silver | Ulrike Urbansky | |
| Bronze | Anika Ahrens |
30 July
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| align=left | align=left | | 56.00 | CR | |||
| align=left | align=left | 56.08 | |||||
| align=left | align=left | 57.34 | |||||
| 4 | align=left | align=left |  | 57.36 | |||
| 5 | align=left | align=left |  | 57.93 | |||
| 6 | align=left | align=left |  | 58.37 | |||
| 7 | align=left | align=left |  | 59.16 | |||
| 8 | align=left | align=left | | 59.34 |
29 July
Qualified: first 2 in each heat and 2 best to the Final
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | 56.66 | Q, CR | |||
| 2 | align=left | align=left | | 58.18 | Q | ||
| 3 | align=left | align=left | | 58.23 | q | ||
| 4 | align=left | align=left | 58.28 | ||||
| 5 | align=left | align=left | 58.32 | ||||
| 6 | align=left | align=left |  | 58.33 | |||
| 7 | align=left | align=left |  | 59.39 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 58.11 | Q | ||
| 2 | align=left | align=left | | 58.37 | Q | ||
| 3 | align=left | align=left | 58.44 | ||||
| 4 | align=left | align=left |  | 58.92 | |||
| 5 | align=left | align=left | | 59.60 | |||
| 6 | align=left | Beatriz Moreno | align=left |  | 60.65 |
| Rank | Name | Nationality | Time | Notes | |||
|---|---|---|---|---|---|---|---|
| 1 | align=left | align=left | | 57.33 | Q | ||
| 2 | align=left | align=left | | 58.21 | Q | ||
| 3 | align=left | align=left | 58.21 | q | |||
| 4 | align=left | align=left | 58.90 | ||||
| 5 | align=left | align=left | | 58.94 | |||
| 6 | align=left | align=left | 60.60 |
According to an unofficial count, 19 athletes from 14 countries participated in the event.
(1) (1) (1) (3) (1) (2) (1) (1) (1)