| Competition: | Primera B Nacional |
| Season: | 1999–00 |
| Winners: | Huracán (2nd divisional title) |
| Promoted: | Huracán Los Andes Almagro |
| Relegated: | Deportivo Español Gimnasia y Tiro Argentino (R) Deportivo Morón Aldosivi Temperley |
| League Topscorer: | Gastón Casas 30 goals |
| Prevseason: | 1998–99 |
| Nextseason: | 2000–01 |
The 1999–00 Argentine Primera B Nacional was the 14th season of second division professional of football in Argentina. 34 teams competed; the champion and runner-up were promoted to Argentine Primera División.
The Promotion Playoff was played by the teams placed 1st and 2nd of each zone. The winning team was declared champion and was automatically promoted to Primera División. The teams that lost in semifinal joined into the Quarterfinals of the Second Promotion Playoff, and the team that lost in the final joined in the semifinal of the Second Promotion Playoff.
|-!colspan="5"|Semifinals|-!colspan="5"|Semifinals1: Qualified because of sport advantage.
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Note: Huracán won 2–1 on aggregate, promoting to Primera DivisiónThe Second Promotion Playoff or Torneo Reducido was played by the teams placed 3rd to 6th of each zone. Atlético de Rafaela and San Martín (M) joined in the Quarterfinals, and Quilmes joined in the Semifinals. The winner was promoted to Primera División.
1: Qualified because of sport advantage.
The best two teams of the overall standings that were not promoted (Quilmes and Almagro) played against the 18th and the 17th placed of the Relegation Table of 1999–2000 Primera División.
|-!colspan="5"|Relegation/promotion playoff 1|-!colspan="5"|Relegation/promotion playoff 2|-|}
Note: Clubs with indirect affiliation with AFA are relegated to the Torneo Argentino A, while clubs directly affiliated face relegation to Primera B Metropolitana. Clubs with direct affiliation are all from Greater Buenos Aires, with the exception of Newell's, Rosario Central, Central Córdoba and Argentino de Rosario, all from Rosario, and Unión and Colón from Santa Fe.
| width=28 | width=185 | Team | width=50 | width=50 | width=50 | width=50 | width=50 | width=50 | Situation | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Atlético de Rafaela | 45 | 44 | 51 | 140 | 90 | Indirect | ||||||||||
| 2 | — | 46 | 46 | 92 | 60 | ||||||||||||
| 3 | Independiente Rivadavia | — | — | 45 | 45 | 30 | |||||||||||
| 4 | San Martín (SJ) | 48 | 36 | 49 | 133 | 90 | |||||||||||
| 5 | Atlético Tucumán | 43 | 57 | 29 | 129 | 90 | |||||||||||
| 6 | Almirante Brown (A) | 45 | 42 | 40 | 127 | 90 | |||||||||||
| 7 | — | 37 | 47 | 84 | 60 | ||||||||||||
| 8 | Villa Mitre | — | — | 41 | 41 | 30 | |||||||||||
| 9 | San Martín (T) | 43 | 51 | 52 | 121 | 90 | |||||||||||
| 10 | San Martín (M) | 50 | 57 | 36 | 116 | 90 | |||||||||||
| 11 | Godoy Cruz | 50 | 57 | 36 | 115 | 90 | |||||||||||
| 12 | Racing (C) | — | — | 38 | 38 | 30 | |||||||||||
| 13 | Olimpo | 29 | 43 | 41 | 113 | 90 | |||||||||||
| 14 | Cipolletti | 34 | 53 | 23 | 110 | 90 | |||||||||||
| 15 | Gimnasia y Tiro | — | 33 | 40 | 73 | 60 | Torneo Argentino A | ||||||||||
| 16 | Aldosivi | 36 | 42 | 31 | 109 | 90 |
| width=28 | width=185 | Team | width=50 | width=50 | width=50 | width=50 | width=50 | width=50 | Situation | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Huracán | — | — | 64 | 64 | 34 | Direct | ||||||||||
| 2 | Banfield | 62 | 43 | 59 | 164 | 96 | |||||||||||
| 3 | Arsenal | 46 | 57 | 55 | 158 | 96 | |||||||||||
| 4 | Quilmes | 48 | 47 | 63 | 158 | 96 | |||||||||||
| 5 | El Porvenir | — | 45 | 50 | 95 | 66 | |||||||||||
| 6 | Defensa y Justicia | 42 | 44 | 52 | 138 | 96 | |||||||||||
| 7 | Los Andes | 48 | 28 | 63 | 138 | 96 | |||||||||||
| 8 | Central Córdoba (R) | 48 | 43 | 38 | 129 | 96 | |||||||||||
| 9 | Tigre | — | 43 | 45 | 88 | 66 | |||||||||||
| 10 | Platense | — | — | 45 | 45 | 34 | |||||||||||
| 11 | All Boys | 46 | 51 | 29 | 126 | 96 | |||||||||||
| 12 | San Miguel | 45 | 43 | 35 | 123 | 96 | |||||||||||
| 13 | Almagro | 22 | 36 | 62 | 120 | 96 | |||||||||||
| 14 | Nueva Chicago | 40 | 44 | 33 | 117 | 96 | |||||||||||
| 15 | — | 43 | 37 | 80 | 66 | Primera B Metropolitana | |||||||||||
| 16 | Argentino (R) | — | — | 30 | 30 | 34 | |||||||||||
| 17 | Deportivo Morón | 30 | 28 | 22 | 80 | 96 | |||||||||||
| 18 | Temperley | — | — | 25 | 25 | 34 |