| Competition: | Tunisian Ligue Professionnelle 1 |
| Season: | 1999–2000 |
| Winners: | Espérance de Tunis |
| Relegated: | CO Transports AS Marsa |
| Continentalcup1: | Champions League |
| Continentalcup1 Qualifiers: | Espérance de Tunis |
| Continentalcup2: | Cup Winners' Cup |
| Continentalcup2 Qualifiers: | Club Africain |
| Continentalcup3: | CAF Cup |
| Continentalcup3 Qualifiers: | Étoile du Sahel |
| League Topscorer: | Ali Zitouni (19 goals) |
| Biggest Home Win: | OB 5-0 ESZ EST 5-0 ASM ESS 5-0 JSK |
| Biggest Away Win: | CSS 0-4 EST USM 0-4 EST OB 0-4 ST |
| Highest Scoring: | COT 2-5 ASM |
| Matches: | 132 |
| Total Goals: | 304 |
| Prevseason: | 1998–99 |
| Nextseason: | 2000–01 |
The 1999–2000 Tunisian Ligue Professionnelle 1 season was the 74th season of top-tier football in Tunisia.