| Competition: | Midland Football Alliance |
| Season: | 1999–2000 |
| Winners: | Oadby Town |
| Relegated: | Pershore Town |
| Matches: | 462 |
| Total Goals: | 1452 |
| Prevseason: | 1998–99 |
| Nextseason: | 2000–01 |
The 1999–2000 Midland Football Alliance season was the sixth in the history of Midland Football Alliance, a football competition in England.
The league featured 19 clubs from the previous season, along with three new clubs: