| Season: | 1999–2000 |
| Continentalcup2 Qualifiers: | Drnovice Liberec (via Domestic Cup) |
| League Topscorer: | Vratislav Lokvenc (22) |
| Matches: | 240 |
| Total Goals: | 570 |
| Average Attendance: | 5,972[2] |
| Nextseason: | 2000–01 |
The 1999–2000 Czech First League, known as the Gambrinus liga for sponsorship reasons, was the seventh season of top-tier football in the Czech Republic.
| Rank | Player | Club | Goals |
|---|---|---|---|
| 1 |  Vratislav Lokvenc | Sparta Prague | 22 |
| 2 | Marek Kincl | Slovan Liberec / Viktoria Žižkov | 16 |
| 3 | Stanislav Vlček | Sigma Olomouc | 13 |
| 4 | Vítězslav Tuma | Drnovice | 11 |
| Tomáš Janda | České Budějovice | ||
| Tomáš Došek | Slavia Prague | ||
| Horst Siegl | Sparta Prague |