| Season: | 1999–2000 |
| League Topscorer: |  René Mendieta (15) |
| Matches: | 110 |
| Total Goals: | 302 |
| Nextseason: | 2000–01 |
The ANAPROF 1999–2000 season was the twelfth season since its establishment. It began on August 1, 1999, and ended on January 30, 2000. 10 teams competed in the league, 9 of which returned from the previous season and 1 of which was promoted from Primera A.
Tauro were crowned champions after defeating Plaza Amador 2-0 in the Rommel Fernández becoming Tauro's fifth title in its history. After clinching this title Tauro earned the Panama 1 spot in the 2001 season of the Copa Interclubes UNCAF while Plaza Amador earned the Panama 2 spot as the runner-up.
Previous to the start of the season there was a championship that went on from April to July amongst the participating teams to determine who would play in the 2000 season of the Copa Interclubes UNCAF; the 1999–2000 Campeonato Apertura. Panamá Viejo was crowned champion on July 2 after defeating Tauro 2-0.
| Club | City | Stadium | 1998-99 season |
|---|---|---|---|
| Árabe Unido | Colón | Estadio Mariano Bula | 2nd in ANAPROF (champion) |
| Atlético Nacional | Panama City | Estadio Agustín Sánchez | 6th in ANAPROF (hexagonal) |
| Chiriquí F.C. | David | Estadio Kenny Serracin Estadio San Cristóbal | 8th in ANAPROF |
| Atlético Municipal Colón | Colón | Estadio Mariano Bula | Primera A champion |
| Eurokickers F.C. | Panama City | Estadio Rommel Fernández Estadio Javier Cruz | 9th in ANAPROF |
| Panamá Viejo F.C. | Panama City | Estadio Rommel Fernández | 4th in ANAPROF (hexagonal) |
| Plaza Amador | Panama City | Estadio Rommel Fernández | 5th in ANAPROF (Runner-up) |
| San Francisco F.C. | La Chorrera | Estadio Agustín Sánchez | 7th in ANAPROF |
| Sporting '89 | San Miguelito | Estadio Rommel Fernández Estadio Javier Cruz | 1st in ANAPROF |
| Tauro F.C. | Panama City | Estadio Javier Cruz Estadio Rommel Fernández | Copa Rommel Fernández champion |
Semifinal 1----
Semifinal 2----
El Super Clasico Nacional - Tauro v Plaza Amador----------------