| Score: | 6–4, 3–6, 6–3 |
| Draw: | 128 (8 / 8) |
| Seeds: | 16 |
| Before Name: | Wimbledon Championships – Women's singles |
| After Name: | Wimbledon Championships – Women's singles |
See main article: 1994 Wimbledon Championships.
Conchita Martínez defeated Martina Navratilova in the final, 6–4, 3–6, 6–3 to win the ladies' singles tennis title at the 1994 Wimbledon Championships.[1] It was her first and only major title. It was Navratilova's 34th and last major singles final.
Steffi Graf was the three-time defending champion, but lost in the first round to Lori McNeil, ending her streak of 31 consecutive major quarterfinals, dating back to the 1985 US Open. This also marked the first time in the Open Era that a defending major champion lost in the first round of their title defense.[2]
See also: 1 and 1. Steffi Graf (first round)
See also: 2 and 8. 
Arantxa Sánchez Vicario (fourth round)
See also: 3 and 3. Conchita Martínez (champion)
See also: 4 and 6. 
Martina Navratilova (final)
See also: 5 and 5. 
Jana Novotná (quarterfinals)
See also: 6 and 2. 
Kimiko Date (third round)
See also: 7. 
Mary Pierce (withdrew)
See also: 8 and 7. 
Natasha Zvereva (first round)
See also: 9 and 4. Lindsay Davenport (quarterfinals)
See also: 10 and 4. 
Gabriela Sabatini (fourth round)
See also: 11 and 5. Mary Joe Fernández (third round)
See also: 12 and 3. Anke Huber (second round)
See also: 13 and 8. Zina Garrison-Jackson (quarterfinals)
See also: 14 and 2. Amanda Coetzer (fourth round)
See also: 15 and 1. Sabine Hack (first round)
See also: 16 and 7. Magdalena Maleeva (second round)
See also: 17 and 6. Helena Suková (fourth round)
Mary Pierce withdrew for personal reasons. She was replaced in the draw by the highest-ranked non-seeded player Helena Suková, who became the #17 seed.
See main article: 1994 Wimbledon Championships – Women's singles qualifying.