The 1994–95 season was the 80th season of the Isthmian League, which is an English football competition featuring semi-professional and amateur clubs from London, East and South East England. League consisted of four divisions.
| Competition: | Isthmian League Premier Division |
| Season: | 1994–95 |
| Winners: | Enfield |
| Promoted: | Slough Town |
| Relegated: | Chesham United Marlow Wokingham Town |
| Matches: | 462 |
| Total Goals: | 1513 |
| Highest Attendance: | 1,459 – Enfield – Walton & Hersham, (29 April) |
| Attendance: | 207,870 |
| Average Attendance: | 450 (+3.0% to previous season) |
| Prevseason: | 1993–94 |
| Nextseason: | 1995–96 |
The Premier Division consisted of 22 clubs, including 18 clubs from the previous season and four new clubs:
| Competition: | Isthmian League Division One |
| Season: | 1994–95 |
| Winners: | Boreham Wood |
| Promoted: | Boreham Wood Chertsey Town Worthing |
| Relegated: | Dorking Newbury Town Wivenhoe Town |
| Matches: | 462 |
| Total Goals: | 1534 |
| Highest Attendance: | 2,920 – Aldershot Town – Newbury Town, (6 May) |
| Attendance: | 132,267 |
| Average Attendance: | 286 (+68.2% to previous season) |
| Prevseason: | 1993–94 |
| Nextseason: | 1995–96 |
Division One consisted of 22 clubs, including 16 clubs from the previous season and six new clubs:
Clubs relegated from the Premier Division:
Clubs promoted from Division Two:
At the end of the season Leyton who merged with Walthamstow Pennant and formed new club Leyton Pennant.
| Competition: | Isthmian League Division Two |
| Season: | 1994–95 |
| Winners: | Thame United |
| Promoted: | Barton Rovers Oxford City Thame United |
| Relegated: | Aveley Windsor & Eton |
| Matches: | 462 |
| Total Goals: | 1528 |
| Highest Attendance: | 578 – Thame United – Oxford City, (26 December) |
| Attendance: | 45,993 |
| Average Attendance: | 100 (-55.9% to previous season) |
| Prevseason: | 1993–94 |
| Nextseason: | 1995–96 |
Division Two consisted of 22 clubs, including 16 clubs from the previous season and six new clubs:
Clubs relegated from Division One:
Clubs promoted from the Third Division:
At the end of the season Malden Vale resigned from the league and merged into Raynes Park to form a new club Raynes Park Vale, who joined Combined Counties League.
| Competition: | Isthmian League Division Three |
| Season: | 1994–95 |
| Winners: | Collier Row |
| Promoted: | Bedford Town Canvey Island Collier Row |
| Matches: | 420 |
| Total Goals: | 1377 |
| Highest Attendance: | 570 – Collier Row – Canvey Island, (19 April) |
| Attendance: | 47,430 |
| Average Attendance: | 113 (+27.0% to previous season) |
| Prevseason: | 1993–94 |
| Nextseason: | 1995–96 |
Division Three consisted of 21 clubs, including 17 clubs from the previous season and four new clubs:
At the end of the season Feltham & Hounslow resigned from the league and joined the Combined Counties League. Also, club was renamed back into Feltham.