The 1993–94 season was the 79th season of the Isthmian League, which is an English football competition featuring semi-professional and amateur clubs from London, East and South East England. League consisted of four divisions.
| Competition: | Isthmian League Premier Division |
| Season: | 1993–94 |
| Winners: | Stevenage Borough |
| Promoted: | Stevenage Borough |
| Relegated: | Basingstoke Town Dorking Wivenhoe Town |
| Matches: | 462 |
| Total Goals: | 1432 |
| Highest Attendance: | 3,005 – Stevenage Borough – Harrow Borough, (7 May) |
| Attendance: | 201,909 |
| Average Attendance: | 437 (-5.2% to previous season) |
| Prevseason: | 1992–93 |
| Nextseason: | 1994–95 |
The Premier Division consisted of 22 clubs, including 19 clubs from the previous season and three clubs promoted from Division One:
| Competition: | Isthmian League Division One |
| Season: | 1993–94 |
| Winners: | Bishop's Stortford |
| Promoted: | Bishop's Stortford Purfleet Walton & Hersham |
| Relegated: | Chalfont St Peter Croydon Windsor & Eton |
| Matches: | 462 |
| Total Goals: | 1421 |
| Highest Attendance: | 890 – Worthing – Bognor Regis Town, (4 April) |
| Attendance: | 78,717 |
| Average Attendance: | 170 (+4.9% to previous season) |
| Prevseason: | 1992–93 |
| Nextseason: | 1994–95 |
Division One consisted of 22 clubs, including 16 clubs from the previous season and six new clubs:
Clubs relegated from the Premier Division:
Clubs promoted from Division Two:
| Competition: | Isthmian League Division Two |
| Season: | 1993–94 |
| Winners: | Newbury Town |
| Promoted: | Aldershot Town Chertsey Town Newbury Town |
| Relegated: | Collier Row Lewes |
| Matches: | 462 |
| Total Goals: | 1392 |
| Highest Attendance: | 3,301 – Aldershot Town – Newbury Town, (29 March) |
| Attendance: | 105,048 |
| Average Attendance: | 227 (+81.6% to previous season) |
| Prevseason: | 1992–93 |
| Nextseason: | 1994–95 |
Division Two consisted of 22 clubs, including 17 clubs from the previous season and five new clubs:
Clubs relegated from Division One:
Clubs promoted from Division Three:
| Competition: | Isthmian League Division Three |
| Season: | 1993–94 |
| Winners: | Bracknell Town |
| Promoted: | Bracknell Town Cheshunt Oxford City |
| Matches: | 420 |
| Total Goals: | 1234 |
| Highest Attendance: | 646 – Leighton Town – Tring Town, (27 December) |
| Attendance: | 37,412 |
| Average Attendance: | 89 (-64.0% to previous season) |
| Prevseason: | 1992–93 |
| Nextseason: | 1994–95 |
Division Three consisted of 21 clubs, including 16 clubs from the previous season and five new clubs: