See main article: 1991 Lipton International Players Championships.
Defending champion Monica Seles defeated Gabriela Sabatini in the final, 6–3, 7–5 to win the women's singles tennis title at the 1991 Miami Open.
All seeds received a bye into the second round.
See also: 1 and 1. Steffi Graf (semifinals)
See also: 2 and 8. Monica Seles (champion)
See also: 3 and 3. 
Gabriela Sabatini (final)
See also: 4 and 6. 
Mary Joe Fernández (semifinals)
See also: 5 and 4. Zina Garrison-Jackson (quarterfinals)
See also: 6 and 7. Jennifer Capriati (quarterfinals)
See also: 7 and 2. Manuela Maleeva-Fragniere (quarterfinals)
See also: 8 and 5. Natasha Zvereva (second round)
See also: 9 and 7. 
Barbara Paulus (fourth round)
See also: 10 and 3. 
Nathalie Tauziat (fourth round)
See also: 11 and 1. 
Helen Kelesi (second round)
See also: 12 and 5. Amy Frazier (third round)
See also: 13 and 6. 
Laura Gildemeister (third round)
See also: 14 and 4. Rosalyn Fairbank-Nideffer (second round)
See also: 15 and 8. 
Naoko Sawamatsu (third round)
See also: 16 and 2. 
Raffaella Reggi (fourth round)
See also: 17 and 6. Lori McNeil (third round)
See also: 18 and 3. Manon Bollegraf (second round)
See also: 19 and 2. 
Catarina Lindqvist (second round)
See also: 20 and 8. Susan Sloane (second round)
See also: 21 and 1. Gretchen Magers (third round)
See also: 22 and 5. Regina Rajchrtová (fourth round)
See also: 23 and 2. Meredith McGrath (third round)
See also: 24 and 8. Carrie Cunningham (second round)
See also: 25 and 3. 
Julie Halard (second round)
See also: 26 and 7. Ann Grossman (third round)
See also: 27 and 1. Marianne Werdel (fourth round)
See also: 28 and 6. Cathy Caverzasio (second round)
See also: 29 and 4. Wiltrud Probst (second round)
See also: 30 and 5. Eva Švíglerová (third round)
See also: 31 and 7. Stephanie Rehe (second round)
See also: 32 and 4. Elizabeth Smylie (third round)
See also: 1. Camille Benjamin (Promoted to Main Draw)
See also: 2 and 8. Renata Baranski (second round)
See also: 3 and 6. Bettina Fulco (second round)
See also: 4 and 3. Nathalie Guerrée (first round)
See also: 5 and 5. 
Clare Wood (qualified)
See also: 6 and 4. Eva Pfaff (second round)
See also: 7 and 7. Maïder Laval (qualifying competition)
See also: 8 and 2. Michelle Jaggard (second round)
See also: 9 and 9. 
Sandra Wasserman (qualified)
See also: 10 and 1. Hu Na (first round)
See also: 11 and 7. 
Cláudia Chabalgoity (second round)
See also: 12 and 4. 
Claudine Toleafoa (qualified)
See also: 13 and 8. Noëlle van Lottum (first round)
See also: 14 and 2. Pascale Etchemendy (first round)
See also: 15 and 3. Andrea Vieira (first round)
See also: 16 and 6. Julie Richardson (qualifying competition)
Rene Simpson-Alter Stacey Schefflin Nathalie Housset Claudine Toleafoa Clare Wood
Yael Segal Miriam Oremans
Petra Thorén