| Score: | 6–4, 6–1 |
| Draw: | 128 (8 / 8) |
| Seeds: | 16 |
| Before Name: | Wimbledon Championships – Women's singles |
| After Name: | Wimbledon Championships – Women's singles |
See main article: 1990 Wimbledon Championships.
Martina Navratilova defeated Zina Garrison in the final, 6–4, 6–1 to win the ladies' singles tennis title at the 1990 Wimbledon Championships.[1] It was her ninth Wimbledon singles title and 18th and final major singles title overall, tying Chris Evert's Open Era record. It was also her ninth consecutive Wimbledon final.
Steffi Graf was the two-time defending champion, but lost in the semifinals to Garrison. This marked the first time since the 1987 Australian Open that Graf did not reach a major final, ending a record of 13 consecutive appearances.
By defeating Monica Seles in the quarterfinals, Garrison ended the former's 36-match winning streak, extending back to Miami earlier that year.
See also: 1 and 1. Steffi Graf (semifinals)
See also: 2 and 8. 
Martina Navratilova (champion)
See also: 3 and 3. Monica Seles (quarterfinals)
See also: 4 and 6. 
Gabriela Sabatini (semifinals)
See also: 5 and 4. Zina Garrison (final)
See also: 6 and 5. 
Arantxa Sánchez Vicario (first round)
See also: 7 and 7. Katerina Maleeva (quarterfinals)
See also: 8 and 2. Manuela Maleeva-Fragnière (first round)
See also: 9. Mary Joe Fernández (withdrew)
See also: 10 and 4. Helena Suková (fourth round)
See also: 11 and 5. Natasha Zvereva (quarterfinals)
See also: 12 and 1. Jennifer Capriati (fourth round)
See also: 13 and 2. Jana Novotná (quarterfinals)
See also: 14 and 8. 
Judith Wiesner (fourth round)
See also: 15 and 6. Rosalyn Fairbank (second round)
See also: 16 and 3. Barbara Paulus (first round)
Mary Joe Fernández withdrew due to a knee injury. She was replaced in the draw by lucky loser Anna Ivan.
See main article: 1990 Wimbledon Championships – Women's singles qualifying.