| Year: | 1990 |
| Team: | Eastern Kentucky Colonels |
| Sport: | football |
| Conference: | Ohio Valley Conference |
| Short Conf: | OVC |
| Record: | 10–2 |
| Conf Record: | 5–1 |
| Head Coach: | Roy Kidd |
| Hc Year: | 27th |
| Stadium: | Hanger Field |
| Champion: | OVC co-champion |
| Bowl: | NCAA Division I-AA First Round |
| Bowl Result: | L 17–45 vs. Furman |
The 1990 Eastern Kentucky Colonels football team represented Eastern Kentucky University as a member of the Ohio Valley Conference (OVC) during the 1990 NCAA Division I-AA football season. Led by 27th-year head coach Roy Kidd, the Colonels compiled an overall record of 10–2, with a mark of 5–1 in conference play, and finished as OVC co-champion. Eastern Kentucky advanced to the NCAA Division I-AA First Round and were defeated by Furman.