| Champ: |  Gabriela Sabatini |
| Runner: | Steffi Graf |
| Score: | 3–6, 6–3, 7–5 |
| Type: | singles doubles |
| Draw: | 56 (7) |
| Seeds: | 16 |
| Before Name: | Bausch & Lomb Championships – Singles |
| After Name: | Bausch & Lomb Championships – Singles |
| Main Name: | Amelia Island Championships |
Martina Navratilova was the defending champion but lost in the semifinals to Gabriela Sabatini.
Sabatini won in the final 3–6, 6–3, 7–5 against Steffi Graf.
A champion seed is indicated in bold text while text in italics indicates the round in which that seed was eliminated. The top eight seeds received a bye to the second round.
Martina Navratilova (semifinals) Gabriela Sabatini (champion) Lori McNeil (second round)
Arantxa Sánchez (semifinals)
Hana Mandlíková (quarterfinals)
Sandra Cecchini (second round) Bettina Fulco (second round)
Judith Wiesner (quarterfinals) Terry Phelps (second round)