| Score: | 7–6(7–1), 6–3 |
| Draw: | 128 (8 / 8) |
| Seeds: | 16 |
| Before Name: | Wimbledon Championships – Women's singles |
| After Name: | Wimbledon Championships – Women's singles |
See main article: 1986 Wimbledon Championships. Four-time defending champion Martina Navratilova defeated Hana Mandlíková in the final, 7–6(7–1), 6–3 to win the ladies' singles tennis title at the 1986 Wimbledon Championships.[1] It was her seventh Wimbledon singles title and 14th major singles title overall. Mandlíková was attempting to complete the career Grand Slam.
This marked the first Wimbledon appearance of future champion Jana Novotná. She was defeated by Susan Mascarin in the first round.
See also: 1 and 1. 
Martina Navratilova (champion)
See also: 2 and 8. Chris Evert Lloyd (semifinals)
See also: 3 and 6. Hana Mandlíková (final)
See also: 4 and 3. Claudia Kohde-Kilsch (third round)
See also: 5 and 5. Pam Shriver (first round)
See also: 6 and 4. Kathy Rinaldi (first round)
See also: 7 and 7. Helena Suková (quarterfinals)
See also: 8 and 2. Manuela Maleeva (fourth round)
See also: 9 and 7. Zina Garrison (second round)
See also: 10 and 3. 
Gabriela Sabatini (semifinals)
See also: 11 and 6. 
Carling Bassett (fourth round)
See also: 12 and 2. Stephanie Rehe (first round)
See also: 13. Barbara Potter (withdrew)
See also: 14 and 1. 
Wendy Turnbull (first round)
See also: 15 and 4. 
Catarina Lindqvist (quarterfinals)
See also: 16 and 8. Kathy Jordan (fourth round)
Steffi Graf was originally seeded #3 but withdrew due to illness before the tournament draw was made. All original seeds from 4-16 moved up one place, and a new #16 seed was added.
Barbara Potter withdrew due to injury. She was replaced in the draw by lucky loser Ronni Reis.
See main article: 1986 Wimbledon Championships – Women's singles qualifying.