| Year: | 1986 |
| Team: | Eastern Kentucky Colonels |
| Sport: | football |
| Conference: | Ohio Valley Conference |
| Short Conf: | OVC |
| Record: | 10–3–1 |
| Conf Record: | 6–1 |
| Head Coach: | Roy Kidd |
| Hc Year: | 23rd |
| Stadium: | Hanger Field |
| Champion: | OVC co-champion |
| Bowl: | NCAA Division I-AA Semifinal |
| Bowl Result: | L 10–24 at Arkansas State |
The 1986 Eastern Kentucky Colonels football team represented Eastern Kentucky University as a member of the Ohio Valley Conference (OVC) during the 1986 NCAA Division I-AA football season. Led by 23rd-year head coach Roy Kidd, the Colonels compiled an overall record of 10–3–1 with a mark of 6–1 in conference play, sharing the OVC title with Murray State. Eastern Kentucky advanced to the NCAA Division I-AA Championship playoffs, where Colonels defeated Furman in the first round and Eastern Illinois in the quarterfinals before losing to Arkansas State in the semifinals.