| Score: | 6–4, 7–5 |
| Draw: | 96 (10) |
| Seeds: | 8 |
| Before Name: | Wimbledon Championships – Women's singles |
| After Name: | Wimbledon Championships – Women's singles |
| Type: | grand slam |
See main article: 1965 Wimbledon Championships. Margaret Smith defeated the defending champion Maria Bueno in the final, 6–4, 7–5 to win the ladies' singles tennis title at the 1965 Wimbledon Championships.[1] For the first time in the history of seeding in the championships, there was no British player seeded in the draw.
See also: 1 and 1. 
Maria Bueno (final)
See also: 2 and 8. 
Margaret Smith (champion)
See also: 3 and 3. Lesley Turner (quarterfinals)
See also: 4 and 6. 
Nancy Richey (quarterfinals)
See also: 5 and 4. Billie Jean Moffitt (semifinals)
See also: 6 and 5. Carole Graebner (second round)
See also: 7. Annette Van Zyl (withdrew)
See also: 8 and 2. 
Françoise Dürr (fourth round)
Annette Van Zyl withdrew due to a family bereavement. She was replaced in the draw by lucky loser Michelle Boulle.