| Year: | 1965 |
| Team: | Western Kentucky Hilltoppers |
| Sport: | football |
| Conference: | Ohio Valley Conference |
| Short Conf: | OVC |
| Record: | 2–6–2 |
| Conf Record: | 1–5–1 |
| Head Coach: | Nick Denes |
| Hc Year: | 8th |
| Captain: | Ken Frick |
The 1965 Western Kentucky Hilltoppers football team represented Western Kentucky State College (now known as Western Kentucky University) as a member of the Ohio Valley Conference (OVC) during the 1965 NCAA College Division football season. Led by eighth-year head coach Nick Denes, the Hilltoppers compiled an overall record of 2–6–2 with a mark of 1–5–1 in conference play, for seventh place in the OVC. The team's captain was Ken Frick.[1]