| Venue: | San Sebastián, Spain |
| Date: | 2-5 September 1965 |
| Previous: | Sallanches 1964 |
| Next: | Nürburgring 1966 |
The 1965 UCI Road World Championships took place from 2-5 September 1965 in San Sebastián, Spain.
| Race: | Gold: | Time | Silver: | Time | Bronze : | Time | |
| Men | |||||||
| Men's road race | Tom Simpson | 6 h 39 min 19s | Rudi Altig | m.t. | Roger Swerts | + 3 min 40s | |
| Amateurs' road race | Jacques Botherel | - | José Manuel Lasa | - | Battista Monti | - | |
| Team time trial | - | - | - | ||||
| Women | |||||||
| Women's road race | Elisabeth Eichholz | - | Yvonne Reynders | - | Aino Puronen | - | |