| National Challenge Cup | |
| Year: | 1965 |
| Country: |  United States |
| Date: | --> |
| Defending Champions: | Los Angeles Kickers |
| Winners: | New York Ukrainians |
| Count: | 1 |
| Second: | Chicago Hansa |
| Semifinal1: | --> |
| Semifinal2: | --> |
| Prev Season: | 1964 |
| Next Season: | 1966 |
The 1965 National Challenge Cup was the 52nd edition of the USSFA's annual open soccer championship. The New York Ukrainians defeated the Chicago Hansa to win.