| Year: | 1965 |
| Team: | Middle Tennessee Blue Raiders |
| Sport: | football |
| Conference: | Ohio Valley Conference |
| Short Conf: | OVC |
| Record: | 10–0 |
| Conf Record: | 7–0 |
| Head Coach: | Charles M. Murphy |
| Hc Year: | 19th |
| Captain: | K. Atchley, W. Randolph |
| Stadium: | Horace Jones Field |
| Champion: | OVC champion |
The 1965 Middle Tennessee Blue Raiders football team represented Middle Tennessee State University—as a member of the Ohio Valley Conference (OVC) during the 1965 NCAA College Division football season. Led by 19th-year head coach Charles M. Murphy, the Blue Raiders compiled a record an overall record of 10–0 with a mark of 7–0 in conference play, winning the OVC title. The team's captains were K. Atchley and W. Randolph.[1]