| Year: | 1965 |
| Team: | McNeese State Cowboys |
| Sport: | football |
| Conference: | Gulf States Conference |
| Short Conf: | GSC |
| Record: | 5–4 |
| Conf Record: | 4–1 |
| Head Coach: | Les DeVall |
| Hc Year: | 9th |
| Stadium: | Cowboy Stadium |
| Champion: | GSC co-champion |
The 1965 McNeese State Cowboys football team was an American football team that represented McNeese State College (now known as McNeese State University) as a member of the Gulf States Conference (GSC) during the 1965 NCAA College Division football season. In their ninth year under head coach Les DeVall, the team compiled an overall record of 5–4 with a mark of 4–1 in conference play, and finished as GSC co-champion.