| Year: | 1965 |
| Team: | Duke Blue Devils |
| Sport: | football |
| Conference: | Atlantic Coast Conference |
| Short Conf: | ACC |
| Record: | 6–4 |
| Conf Record: | 4–2 |
| Head Coach: | William D. Murray |
| Hc Year: | 15th |
| Mvp: | John McNabb |
| Captain: | John Gutekunst |
| Stadium: | Duke Stadium |
The 1965 Duke Blue Devils football team was an American football team that represented Duke University as a member of the Atlantic Coast Conference (ACC) during the 1965 NCAA University Division football season. In their 15th year under head coach William D. Murray, the Blue Devils complied an overall record of 6–4, with a conference record of 4–2, and finished third in the ACC.