| Competition: | Campeonato Profesional |
| Season: | 1965 |
| Winners: | Deportivo Cali (1st title) |
| Matches: | 312 |
| Total Goals: | 992 |
| League Topscorer: | Perfecto Rodríguez (38 goals) |
| Biggest Home Win: | América de Cali 6–0 Cúcuta Deportivo Deportivo Cali 6–0 Deportes Quindío |
| Biggest Away Win: | Independiente Medellín 1–5 Millonarios Cúcuta Deportivo 1–5 Santa Fe |
| Highest Scoring: | Millonarios 7–4 Independiente Medellín |
| Prevseason: | 1964 |
| Nextseason: | 1966 |
The 1965 Campeonato Profesional was the 18th season of Colombia's top-flight football league. 13 teams competed against one another. Deportivo Cali won their first league title in history, cutting a streak of four straight championships won by Millonarios.
The same 13 teams from the last tournament competed in this one. The tournament was once again played under a round-robin format, with every team playing each other four times (twice at home and twice away) for a total of 48 matches. Teams received two points for a win and one point for a draw. If two or more teams were tied on points, places were determined by goal difference. The team with the most points became the champion of the league. 312 matches were played during the season, with a total of 992 goals scored. Deportivo Cali won the championship for the first time, the runners-up were Atlético Nacional.[1] Santa Fe scored the highest number of goals, with 99 goals scored, Cúcuta Deportivo conceded the most goals with 102 goals against. Argentine player Perfecto Rodríguez, who played for Independiente Medellín, was the season's top goalscorer with 38 goals.[2]
| Rank | Name | Club | Goals | ||
|---|---|---|---|---|---|
| align=center | 1 |  Perfecto Rodríguez | Independiente Medellín | 38 | |
| align=center | 2 |  Jorge Ramírez Gallego | Deportivo Cali | 32 | |
| align=center | 3 | Efraín Padilla | Deportivo Pereira | 30 | |
| align=center | 4 |  Waldir Cardoso | Unión Magdalena | 27 | |
| align=center | 5 | Omar Devani | Santa Fe | 26 | |
| align=center | 6 | Harvey Colonia | América de Cali | 23 | |
| 7 | Sílvio Faria | Millonarios | 21 | ||
| Alfonso Cañón | Santa Fe | 21 | |||
| align=center | 9 | Horacio Di Loreto | Atlético Bucaramanga | 20 |
Source: RSSSF.com Colombia 1965