| Competition: | Campeonato Ecuatoriano de Fútbol |
| Season: | 1965 |
| Winners: | Emelec (3rd title) |
| League Topscorer: | Helio Cruz (8 goals) |
| Continentalcup1: | Copa Libertadores |
| Continentalcup1 Qualifiers: | Emelec 9 de Octubre |
| Biggest Away Win: | El Nacional 0–2 9 de Octubre Aucas 1–3 9 de Octubre Universidad Católica 0–2 Patria |
| Highest Scoring: | Barcelona 5–2 El Nacional |
| Matches: | 33 |
| Total Goals: | 77 |
| Prevseason: | 1964 |
| Nextseason: | 1966 |
The 1965 Campeonato Ecuatoriano de Fútbol (English: Ecuadorian Football Championship) was the 7th national championship for football teams in Ecuador. Emelec won their third national title. They qualified to the 1966 Copa Libertadores along with 9 de Octubre.
The number of teams remained the same at eight. The qualified teams included the top-four finishers from the Campeonato Interandino and the Campeonato de Guayaquil. Universidad Católica made their first appearance in the tournament.
| width=100 | Competition | width=150 | Team | Qualification method |
|---|---|---|---|---|
| Guayaquil 4 berths | Barcelona | 1965 Guayaquil champion | ||
| Emelec | 1965 Guayaquil runner-up | |||
| 9 de Octubre | 1965 Guayaquil top-four finishers | |||
| Patria | ||||
| Interandino 4 berths | Universidad Católica | 1965 Interandino champion | ||
| LDU Quito | 1965 Interandino runner-up | |||
| Aucas | 1965 Interandino top-four finishers | |||
| El Nacional | ||||
Since 9 de Octubre, Barcelona, and Patria finished the tournament equal on points, a playoff was needed to determine who finished as the runner-up and qualified to the 1966 Copa Libertadores. Patria declined to participate. After tying at a goal apiece, 9 de Octubre was declared the runner-up since they had a better goal average throughout the season (1.37 v. 1.36).