| Year: | 1965 |
| Team: | Appalachian State Mountaineers |
| Sport: | football |
| Conference: | Carolinas Conference |
| Short Conf: | Carolinas |
| Record: | 5–5 |
| Conf Record: | 3–3 |
| Head Coach: | Carl Messere |
| Hc Year: | 1st |
| Stadium: | Conrad Stadium |
The 1965 Appalachian State Mountaineers football team was an American football team that represented Appalachian State Teachers College (now known as Appalachian State University) as a member of the Carolinas Conference during the 1965 NAIA football season. In their first year under head coach Carl Messere, the Mountaineers compiled an overall record of 5–5, with a mark of 3–3 in conference play, and finished tied for fourth in the Carolinas Conference.