| Competition: | Midland Football League |
| Season: | 1965–66 |
| Winners: | Worksop Town |
| Matches: | 462 |
| Total Goals: | 1955 |
| Prevseason: | 1964–65 |
| Nextseason: | 1966–67 |
The 1965–66 Midland Football League season was the 66th in the history of the Midland Football League, a football competition in England.
The league featured 20 clubs which competed in the previous season, along with two new clubs, joined from the North Regional League: