| Year: | 1964 |
| Team: | Tennessee A&I Tigers |
| Sport: | football |
| Conference: | Midwest Athletic Association |
| Short Conf: | MAA |
| Record: | 8–2 |
| Conf Record: | 3–0 |
| Head Coach: | John Merritt |
| Hc Year: | 2nd |
| Stadium: | Hale Stadium |
| Champion: | MAA champion |
The 1964 Tennessee A&I Tigers football team represented Tennessee Agricultural & Industrial State College (now known as Tennessee State University) as a member of the Midwest Athletic Association (MAA) during the 1964 NCAA College Division football season. Led by second-year head coach John Merritt, the Tigers compiled an overall record of 8–2, with a 3–0 conference record, and finished as MAA champion.