| Competition: | Segunda División |
| Season: | 1964 |
| Winners: | O'Higgins |
| Promoted: | O'Higgins |
| Relegated: | None |
| Prevseason: | 1963 |
| Nextseason: | 1965 |
The 1964 Segunda División de Chile was the 13th season of the Segunda División de Chile.
O'Higgins was the tournament's champion.[1]