| Competition: | Peruvian Segunda División |
| Season: | 1964 |
| Winners: | Defensor Arica |
| Prevseason: | 1963 |
| Nextseason: | 1965 |
The 1964 Peruvian Segunda División, the second division of Peruvian football (soccer), was played by 10 teams. The tournament winner, Defensor Arica was promoted to the 1965 Peruvian Primera División.