| Competition: | Campeonato Profesional |
| Season: | 1964 |
| Winners: | Millonarios (9th title) |
| Matches: | 312 |
| Total Goals: | 1002 |
| League Topscorer: | Omar Devani (28 goals) |
| Biggest Home Win: | Santa Fe 6–0 Atlético Bucaramanga Atlético Nacional 7–1 Atlético Bucaramanga |
| Biggest Away Win: | Unión Magdalena 1–4 Independiente Medellín Santa Fe 0–3 Deportivo Pereira Santa Fe 1–4 Deportes Tolima |
| Highest Scoring: | Atlético Nacional 4–6 Millonarios América de Cali 7–3 Unión Magdalena |
| Prevseason: | 1963 |
| Nextseason: | 1965 |
The 1964 Campeonato Profesional was the 17th season of Colombia's top-flight football league. 13 teams competed against one another. Millonarios won the league for the ninth time in its history and fourth in a row, defending successfully the title won in the previous three seasons.
The same 13 teams from the last tournament competed in this one. The tournament was once again played under a round-robin format, with every team playing each other four times (twice at home and twice away) for a total of 48 matches. Teams received two points for a win and one point for a draw. If two or more teams were tied on points, places were determined by goal difference. The team with the most points became the champion of the league. Millonarios won the championship for the ninth time. The runners-up were Cúcuta Deportivo.[1]
| Team | City | Stadium | |
|---|---|---|---|
| América de Cali | Cali | Estadio Olímpico Pascual Guerrero | |
| Atlético Bucaramanga | Bucaramanga | Estadio Alfonso López | |
| Atlético Nacional | Medellín | Estadio Atanasio Girardot | |
| Cúcuta Deportivo | Cúcuta | Estadio General Santander | |
| Deportes Quindío | Armenia | Estadio San José de Armenia | |
| Deportes Tolima | Ibagué | Estadio 10 de Mayo | |
| Deportivo Cali | Cali | Estadio Olímpico Pascual Guerrero | |
| Deportivo Pereira | Pereira | Estadio Alberto Mora Mora | |
| Independiente Medellín | Medellín | Estadio Atanasio Girardot | |
| Millonarios | Bogotá | Estadio El Campín | |
| Once Caldas | Manizales | Estadio Fernando Londoño Londoño | |
| Santa Fe | Bogotá | Estadio El Campín | |
| Unión Magdalena | Santa Marta | Estadio Eduardo Santos |
| Rank | Name | Club | Goals | |
|---|---|---|---|---|
| align=center | 1 |  Omar Devani | Atlético Bucaramanga / Unión Magdalena | 28 |
| align=center | 2 |  José Marin | Santa Fe | 27 |
| 3 | José Omar Verdún | Cúcuta Deportivo | 24 | |
| Edgardo López | Deportes Tolima | 24 | ||
| 5 | Perfecto Rodríguez | Independiente Medellín | 22 | |
| Sílvio Faria | Millonarios | 22 | ||
| 7 |  Harvey Colonia | América de Cali | 21 | |
| José Américo Montanini | Atlético Bucaramanga | 21 | ||
| 9 | Juan Eulogio Urriolabeitía | Atlético Nacional | 19 | |
| Efraín Padilla | Deportivo Pereira | 19 |
Source: RSSSF.com Colombia 1964