| Competition: | Serie A |
| Season: | 1964–65 |
| Dates: | 13 September 1964 – 6 June 1965 |
| Winners: | Internazionale 9th title |
| Relegated: | Genoa Messina Mantova |
| Continentalcup1: | European Cup |
| Continentalcup1 Qualifiers: | Internazionale |
| Continentalcup3: | Inter-Cities Fairs Cup |
| Continentalcup3 Qualifiers: | Milan Torino Fiorentina Roma |
| Continentalcup2: | Cup Winners' Cup |
| Continentalcup2 Qualifiers: | Juventus |
| League Topscorer: | Sandro Mazzola Alberto Orlando (17 goals each) |
| Matches: | 306 |
| Total Goals: | 640 |
| Prevseason: | 1963–64 |
| Nextseason: | 1965–66 |
The 1964–65 Serie A season was won by Internazionale.
Varese, Cagliari and Foggia had been promoted from Serie B.
| Rank | Player | Club | Goals |
|---|---|---|---|
| 1 |  Sandro Mazzola | Internazionale | 17 |
| Alberto Orlando | Fiorentina | ||
| 3 |  Amarildo | Milan | 15 |
| 4 | Carlo Facchin | Catania | 13 |
| Harald Nielsen | Bologna | ||
| 6 | Luís Vinício | Vicenza | 12 |
| Giancarlo Danova | Catania | ||
| Paolo Ferrario | Milan | ||
| 9 | Helmut Haller | Bologna | 11 |
| Giampaolo Menichelli | Juventus | ||
| 11 | Giorgio Ferrini | Torino | 10 |
| Luigi Simoni | Torino | ||
| Jair da Costa | Internazionale | ||
| Romano Bagatti | Messina | ||
| Cosimo Nocera | Foggia |