| Competition: | Segunda División |
| Season: | 1964–65 |
| Winners: | Pontevedra Mallorca |
| Promoted: | Pontevedra Mallorca Sabadell Málaga |
| Relegated: | Orense Onteniente Real Unión Abarán |
| League Topscorer: | José María Lizarralde (20 goals) |
| Best Goalkeeper: | Rodri (0.43 goals/match) |
| Biggest Home Win: | (18 October 1964) |
| Biggest Away Win: | (17 January 1965) |
| Highest Scoring: | (11 October 1964) |
| Matches: | 480 |
| Total Goals: | 1258 |
| Prevseason: | 1963–64 |
| Nextseason: | 1965–66 |
The 1964–65 Segunda División season was the 34th since its establishment and was played between 13 September 1964 and 18 April 1965.
32 teams joined the league, including two relegated from the 1963–64 La Liga and 4 promoted from the 1963–64 Tercera División.
| Club | City | Stadium |
|---|---|---|
| CF Badalona | Badalona | Avenida de Navarra |
| Club Baracaldo Altos Hornos | Baracaldo | Lasesarre |
| Burgos CF | Burgos | El Plantío |
| RC Celta de Vigo | Vigo | Balaídos |
| CD Europa | Barcelona | Cerdeña |
| Real Gijón CF | Gijón | El Molinón |
| CD Hospitalet | Hospitalet de Llobregat | Municipal de Deportes |
| SD Indauchu | Bilbao | Garellano |
| UP Langreo | Langreo | Ganzábal |
| CD Orense | Orense | José Antonio |
| Atlético Osasuna | Pamplona | San Juan |
| Pontevedra CF | Pontevedra | Pasarón |
| Real Sociedad | San Sebastián | Atocha |
| Real Unión de Irún | Irun | Stadium Gal |
| CD Sabadell CF | Sabadell | Cruz Alta |
| Real Santander SD | Santander | El Sardinero |
| Goalscorers | Goals | Team |
|---|---|---|
| 20 | Indauchu | |
 Pocholo | 18 | Real Gijón |
| Francisco Solabarrieta | 18 | Real Gijón |
| Alfonso Pérez | 16 | Real Sociedad |
| Abel Fernández | 16 | Racing Santander |
| Goalkeeper | Goals | Matches | Average | Team | |
|---|---|---|---|---|---|
| Rodri | 12 | 28 | Pontevedra | ||
| José Martínez | 27 | 28 | Sabadell | ||
| José María Cobo | 27 | 28 | Real Gijón | ||
| Felipe García | 23 | 21 | Baracaldo | ||
| José Ramón Ibarreche | 34 | 30 | Celta Vigo |
| Club | City | Stadium |
|---|---|---|
| CD Abarán | Abarán | Las Colonias |
| Algeciras CF | Algeciras | El Mirador |
| Cádiz CF | Cádiz | Ramón de Carranza |
| CF Calvo Sotelo | Puertollano | Calvo Sotelo |
| Atlético Ceuta | Ceuta | Alfonso Murube |
| CD Constancia | Inca | Municipal d'Es Cos |
| Granada CF | Granada | Los Cármenes |
| Hércules CF | Alicante | La Viña |
| CD Málaga | Málaga | La Rosaleda |
| RCD Mallorca | Palma de Mallorca | Luis Sitjar |
| Melilla CF | Melilla | Álvarez Claro |
| CD Mestalla | Valencia | Mestalla |
| Onteniente CF | Onteniente | El Clariano |
| Recreativo de Huelva | Huelva | Municipal |
| CD Tenerife | Santa Cruz de Tenerife | Heliodoro Rodríguez López |
| Real Valladolid | Valladolid | José Zorrilla |
| Goalscorers | Goals | Team |
|---|---|---|
| José Martínez | 18 | Valladolid |
| Miguel Sánchez | 17 | Granada |
| Pepillo | 12 | Málaga |
| Juan Ramón Arana | 12 | Hércules |
| 11 | Hércules | |
| Goalkeeper | Goals | Matches | Average | Team | |
|---|---|---|---|---|---|
| José Antonio Omist | 14 | 20 | Algeciras | ||
| José Vicente | 23 | 28 | Mallorca | ||
| Juanito | 22 | 25 | Málaga | ||
| Juan Ignacio Otero | 23 | 23 | Granada | ||
| Antonio Gómez | 26 | 25 | Tenerife |