| Season: | 1964–65 |
| Competition: | Maltese First Division |
| Winners: | Sliema Wanderers F.C. (17th title) |
| Relegated: | Gzira United Rabat |
| Continentalcup1: | European Cup |
| Continentalcup1 Qualifiers: | Sliema Wanderers F.C. |
| Continentalcup2: | European Cup Winners' Cup |
| Continentalcup2 Qualifiers: | Floriana F.C. |
| Matches: | 42 |
| Total Goals: | 168 |
| Prevseason: | 1963–64 |
| Nextseason: | 1965–66 |
The 1964–65 Maltese First Division was the 50th season of top-tier football in Malta. It was contested by 7 teams, and Sliema Wanderers F.C. won the championship.