| Competition: | Cypriot First Division |
| Season: | 1964–65 |
| Prevseason: | 1963–64 |
| Nextseason: | 1965–66 |
The 1964–65 Cypriot First Division was the 26th season of the Cypriot top-level football league.
It was contested by 11 teams, and APOEL F.C. won the championship.