| Competition: | Regionalliga |
| Season: | 1963–64 |
| Winners: | FC St. Pauli SC Tasmania 1900 Berlin Alemannia Aachen Borussia Neunkirchen KSV Hessen Kassel |
| Promoted: | Borussia Neunkirchen Hannover 96 |
| Relegated: | VfL Oldenburg HSV Barmbek-Uhlenhorst SC Union 06 Berlin SpVgg Herten VfB Bottrop Sportfreunde Siegen TuS Duisburg 48/99 Lüner SV ASV Landau SV Niederlahnstein SpVgg 03 Neu-Isenburg Borussia Fulda BC Augsburg Amicitia Viernheim |
| Prevseason: | 1962–63 (2. Oberliga) |
| Nextseason: | 1964–65 |
The 1963–64 Regionalliga was the inaugural Regionalliga season. The league operated in five regional divisions, Berlin, North, South, Southwest and West. The five league champions and the runners-up from the west, south, southwest and north then entered a promotion play-off to determine the two clubs to move up to the Bundesliga for the next season. Southwest champions Borussia Neunkirchen and northern runners-up Hannover 96 were promoted.
The inaugural 1963–64 season saw 18 clubs in the league, the 13 clubs from the Oberliga Nord that did not qualify for the Bundesliga and five from the Northern German Amateurligas.
The inaugural 1963–64 season saw ten clubs in the league, seven clubs from the Oberliga Berlin and three from the Amateurliga Berlin.
The inaugural 1963–64 season saw 20 clubs in the league, the eleven clubs from the Oberliga West that did not qualify for the Bundesliga, eight from the 2. Oberliga West and one club from the Verbandsliga Westfalen, the Lüner SV.
The inaugural 1963–64 season saw 20 clubs in the league, the 14 clubs from the Oberliga Südwest that did not qualify for the Bundesliga and six from the 2. Oberliga Südwest.
The inaugural 1963–64 season saw 20 clubs in the league, the eleven clubs from the Oberliga Süd that did not qualify for the Bundesliga and nine from the 2. Oberliga Süd.
The runners-up of the Regionalliga West and Regionalliga Südwest played a two-leg decider to determined which team qualified for the group stage, which FK Pirmasens won on aggregate.