| Score: | 8–6, 6–0 |
| Draw: | 96 (10) |
| Seeds: | 8 |
| Type: | grand slam |
See main article: 1960 Wimbledon Championships. Maria Bueno successfully defended her title, defeating Sandra Reynolds in the final, 8–6, 6–0 to win the ladies' singles tennis title at the 1960 Wimbledon Championships.
See also: 1 and 1. 
Maria Bueno (champion)
See also: 2 and 8. 
Darlene Hard (quarterfinals)
See also: 3 and 3. 
Christine Truman (semifinals)
See also: 4 and 6. Ann Haydon (semifinals)
See also: 5 and 2. Angela Mortimer (quarterfinals)
See also: 6 and 5. 
Zsuzsa Körmöczy (second round)
See also: 7 and 4. 
Jan Lehane (withdrew)
See also: 8 and 7. Sandra Reynolds (final)
Jan Lehane withdrew due to injury. Her opponent in the first round received a walkover into the second round.