The 1960 European Nations' Cup round of 16 was the second round of the qualifying competition for the 1960 European Nations' Cup. It was contested by Czechoslovakia, the preliminary round winners, along with fifteen other countries that had received a bye. The winners of each of eight home and away ties entered the quarter-finals. The matches were played between September 1958 and October 1959.
See main article: 1960 European Nations' Cup qualifying preliminary round.
The Republic of Ireland and Czechoslovakia faced each other in a two-legged tie, with Czechoslovakia winning and progressing to the round of 16.
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The eight matches took place over two legs, taking place in 1958 and 1959. All times are CET (UTC+1).
Soviet Union won 4–1 on aggregate and advanced to the quarter-finals.----France won 8–2 on aggregate and advanced to the quarter-finals.----Romania won 3–2 on aggregate and advanced to the quarter-finals.----Austria won 6–2 on aggregate and advanced to the quarter-finals.----Yugoslavia won 3–1 on aggregate and advanced to the quarter-finals.----Portugal won 5–2 on aggregate and advanced to the quarter-finals.----Spain won 7–2 on aggregate and advanced to the quarter-finals.----Czechoslovakia won 7–3 on aggregate and advanced to the quarter-finals.