| Year: | 1959 |
| Team: | Cal Poly Mustangs |
| Sport: | football |
| Conference: | California Collegiate Athletic Association |
| Short Conf: | CCAA |
| Record: | 6–3 |
| Conf Record: | 3–2 |
| Head Coach: | LeRoy Hughes |
| Hc Year: | 10th |
| Stadium: | Mustang Stadium |
The 1959 Cal Poly Mustangs football team represented California Polytechnic State College—now known as California Polytechnic State University, San Luis Obispo—as a member of the California Collegiate Athletic Association (CCAA) during the 1959 college football season. Led by tenth-year head coach LeRoy Hughes, Cal Poly compiled an overall record of 6–3 with a mark of 3–2 in conference play, tying for second place in the CCAA. The Mustangs played home games at Mustang Stadium in San Luis Obispo, California.