First-seeded Maureen Connolly defeated Julia Sampson 6–3, 6–2 in the final to win the women's singles tennis title at the 1953 Australian Championships.
The seeded players are listed below. Maureen Connolly is the champion; others show the round in which they were eliminated.
Maureen Connolly (champion) Julia Sampson (finalist)
Helen Angwin (quarterfinals) Mary Hawton (semifinals) Beryl Penrose (quarterfinals) Pam Southcombe (quarterfinals) Alison Baker (second round) Dorn Fogarty (semifinals) Nell Hall Hopman (quarterfinals) Gwen Thiele (second round)