First-seeded Nancye Wynne defeated Thelma Coyne 5–7, 6–4, 6–0 in the final to win the women's singles tennis title at the 1940 Australian Championships.
The seeded players are listed below. Nancye Wynne is the champion; others show the round in which they were eliminated.
Nancye Wynne (champion) Thelma Coyne (finalist) Nell Hopman (semifinals) May Hardcastle (quarterfinals) Joan Hartigan (semifinals) Alison Hattersley (quarterfinals) Gwen O'Halloran (quarterfinals) Constance Coate (quarterfinals)Notes
References