First-seeded Dorothy Round defeated Nancy Lyle 1–6, 6–1, 6–3, in the final to win the women's singles tennis title at the 1935 Australian Championships. The final was the first not to feature an Australian player and is the only all British final in the championship's history.
The seeded players are listed below. Dorothy Round is the champion; others show the round in which they were eliminated.
Dorothy Round (champion)
Emily Hood Westacott (semifinals) Nancy Lyle (finalist) Evelyn Dearman (quarterfinals) Louie Bickerton (quarterfinals) Nell Hopman (semifinals) May Blick (quarterfinals)