| Year: | 1929 |
| Team: | Tuskegee Golden Tigers |
| Sport: | football |
| Conference: | Southern Intercollegiate Athletic Conference |
| Short Conf: | SIAC |
| Record: | 9–0 |
| Conf Record: | – |
| Head Coach: | Cleveland Abbott |
| Hc Year: | 7th |
| Champion: | Black college national co-champion SIAC champion |
The 1929 Tuskegee Golden Tigers football team represented the Tuskegee Normal and Industrial Institute—now known as Tuskegee University—as a member of the Southern Intercollegiate Athletic Conference (SIAC) during the 1929 college football season. In their seventh season under head coach Cleveland Abbott, Tuskegee compiled a 9–0 record, won the SIAC championship, shut out seven of 10 opponents, and outscored all opponents by a total of 249 to 45. The team was recognized as the black college national champion.
Ben Stevenson was the team captain.