The 1927–28 season was the 19th in the history of the Isthmian League, an English football competition.
St Albans City were champions for the second season in a row, winning their third Isthmian League title.
| Competition: | Isthmian League |
| Season: | 1927–28 |
| Winners: | St Albans City |
| Matches: | 182 |
| Total Goals: | 794 |
| Prevseason: | 1926–27 |
| Nextseason: | 1928–29 |