| Type: | grand slam |
| Score: | 6–1, 6–3 |
| Draw: | 19 |
| Seeds: | 8 |
| After Name: | Australian Championships – Women's singles |
First-seeded Daphne Akhurst defeated Esna Boyd 6–1, 6–3, in the final to win the women's singles tennis title at the 1926 Australasian Championships.
The seeded players are listed below. Daphne Akhurst is the champion; others show the round in which they were eliminated.
Daphne Akhurst (champion) Esna Boyd (finalist) Sylvia Harper (semifinals) Marjorie Cox (semifinals) Meryl O'Hara Wood (quarterfinals) Kathleen Le Messurier (quarterfinals) Minnie Richardson (quarterfinals) Beryl Turner (quarterfinals)