| Club: | Tranmere Rovers F.C. |
| Season: | 1926–27 |
| Manager: | Bert Cooke |
| Stadium: | Prenton Park |
| League: | Third Division North |
| League Result: | 9th |
| Cup1: | FA Cup |
| Cup1 Result: | First Round |
| Pattern So1: | _2_stripes_white |
| Leftarm1: | 0000FF |
| Body1: | 0000FF |
| Rightarm1: | 0000FF |
| Shorts1: | FFFFFF |
| Socks1: | 003366 |
| Pattern Name1: | Team |
| Prevseason: | 1925–26 |
| Nextseason: | 1927–28 |
Tranmere Rovers F.C. played the 1926–27 season in the Football League Third Division North. It was their sixth season of league football, and they finished 9th of 22. They reached the First Round of the FA Cup.