The 1926–27 season was the 18th in the history of the Isthmian League, an English football competition.
St Albans City were champions, winning their second Isthmian League title.
| Competition: | Isthmian League |
| Season: | 1926–27 |
| Winners: | St Albans City |
| Matches: | 182 |
| Total Goals: | 828 |
| Prevseason: | 1925–26 |
| Nextseason: | 1927–28 |