| Type: | grand slam |
| Score: | 1–6, 8–6, 6–4 |
| Draw: | 26 |
| Seeds: | 6 |
Daphne Akhurst defeated Esna Boyd 1–6, 8–6, 6–4, in the final to win the women's singles tennis title at the 1925 Australasian Championships.
The seeded players are listed below. Daphne Akhurst is the champion; others show the round in which they were eliminated.
Sylvia Harper (semifinals) Daphne Akhurst (champion) Esna Boyd (finalist) Kathleen Le Messurier (first round) Marjorie Cox (quarterfinals) Minnie Richardson (semifinals)