| Year: | 1916 |
| Team: | Transylvania Pioneers |
| Sport: | football |
| Conference: | Southern Intercollegiate Athletic Association |
| Short Conf: | SIAA |
| Record: | 3–3–2 |
| Conf Record: | 2–3–1 |
| Head Coach: | Willis T. Stewart |
| Hc Year: | 4th |
| Stadium: | Thomas Field |
The 1916 Transylvania Pioneers football team represented Transylvania University as a member the Southern Intercollegiate Athletic Association (SIAA) during the 1916 college football season. Led by fourth-year head coach Willis T. Stewart, the team compiled an overall record of 3–3–2, with a mark of 2–3–1 in conference play.