| Competition: | Scottish Division One |
| Season: | 1900–01 |
| Winners: | Rangers 4th title |
| Relegated: | Partick Thistle |
| Matches: | 110 |
| Total Goals: | 385 |
| League Topscorer: | Robert Hamilton (20 goals) |
| Prevseason: | 1899–1900 |
| Nextseason: | 1901–02 |
The 1900–01 Scottish Division One season was won by Rangers by six points over nearest rival Celtic.